CLEC12B Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| IHC-P, WB, E |
---|---|
Primary Accession | Q2HXU8 |
Other Accession | NP_001123470.1, NP_995324.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 31616 Da |
Antigen Region | 249-276 aa |
Gene ID | 387837 |
---|---|
Other Names | C-type lectin domain family 12 member B, Macrophage antigen H, CLEC12B |
Target/Specificity | This CLEC12B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-276 amino acids from the C-terminal region of human CLEC12B. |
Dilution | WB~~1:1000-1:2000 IHC-P~~1:50~100 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | CLEC12B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | CLEC12B {ECO:0000303|PubMed:34310951, ECO:0000312|HGNC:HGNC:31966} |
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Function | Inhibitory receptor postulated to negatively regulate immune and non-immune functions (PubMed:17562706, PubMed:34310951). Upon phosphorylation, recruits SH2 domain-containing PTPN6 and PTPN11 phosphatases to its ITIM motif and antagonizes activation signals (PubMed:17562706, PubMed:34310951). Although it inhibits KLRK1/NKG2D- mediated signaling, it does not bind known ligands of KLRK1/NKG2D and therefore is not its inhibitory counterpart (PubMed:17562706). May limit activation of myeloid cell subsets in response to infection or tissue inflammation (PubMed:17562706). May protect target cells against natural killer cell-mediated lysis (PubMed:17562706). May negatively regulate cell cycle and differentiation of melanocytes via inactivation of STAT3 (PubMed:34310951). |
Cellular Location | Cell membrane; Single-pass type II membrane protein |
Tissue Location | Detected in colon, heart, kidney, liver, lung, mammary gland, ovary, spleen and testis (PubMed:17562706). Expressed in melanocytes (at protein level) (PubMed:34310951) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cell surface receptor that protects target cells against natural killer cell-mediated lysis. Modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases.
References
Hoffmann, S.C., et al. J. Biol. Chem. 282(31):22370-22375(2007)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
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