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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   UGT8 antibody (Center)   

UGT8 antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UGT8 antibody (Center) AP11630c
    Anti-UGT8 antibody (Center) at 1:2000 dilution + mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - UGT8 antibody (Center) AP11630c
    Anti-UGT8 antibody (Center)at 1:2000 dilution + human heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - UGT8 antibody (Center) AP11630c
    UGT8 antibody (Center) (Cat. #AP11630c) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the UGT8 antibody detected the UGT8 protein (arrow).
  • IHC-P - UGT8 antibody (Center) AP11630c
    UGT8 antibody (Center) (Cat. #AP11630c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT8 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q16880
Other Accession Q09426, Q64676, NP_001121646.1, NP_003351.2
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61438 Da
Antigen Region 366-393 aa
Additional Information
Gene ID 7368
Other Names 2-hydroxyacylsphingosine 1-beta-galactosyltransferase, Ceramide UDP-galactosyltransferase, Cerebroside synthase, UDP-galactose-ceramide galactosyltransferase, UGT8, CGT, UGT4
Target/Specificity This UGT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the Central region of human UGT8.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUGT8 antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UGT8
Synonyms CGT, UGT4
Function Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.
Cellular Location Membrane; Single-pass membrane protein
Research Areas
Citations ( 0 )

Background

Galactocerebrosides are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system and are also present in small amounts in kidney. The key enzymatic step in the biosynthesis of galactocerebrosides consists of the transfer of galactose to ceramide catalyzed by UDP-galactose ceramide galactosyltransferase (CGT, EC 2.4.1.45). The enzyme encoded by the CGT gene is the first involved in complex lipid biosynthesis in the myelinating oligodendrocyte.[supplied by OMIM].

References

Dziecedil Giel, P., et al. Br. J. Cancer 103(4):524-531(2010)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
Ruckhaberle, E., et al. J. Cancer Res. Clin. Oncol. 135(8):1005-1013(2009)
Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009)

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$ 295.00
$ 99.00
Cat# AP11630c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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