|Application ||WB, E|
|Other Accession||Q58NQ5, NP_001108106.1, NP_002646.2|
|Calculated MW||55909 Da|
|Antigen Region||8-35 aa|
|Other Names||Zinc finger protein PLAG1, Pleiomorphic adenoma gene 1 protein, PLAG1|
|Target/Specificity||This PLAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-35 amino acids from the N-terminal region of human PLAG1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PLAG1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor whose activation results in up- regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia.|
|Cellular Location||Nucleus Note=Strong nucleolar localization when sumoylation is inhibited|
|Tissue Location||Expressed in fetal tissues such as lung, liver and kidney. Not detected or weak detection in normal adult tissues, but highly expressed in salivary gland with benign or malignant pleiomorphic adenomas with or without 8q12 aberrations, with preferential occurrence in benign tumors|
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Provided below are standard protocols that you may find useful for product applications.
Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene.
Patz, M., et al. Leuk. Lymphoma 51(8):1379-1381(2010)
Declercq, J., et al. Diabetes 59(8):1957-1965(2010)
Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)
Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010)
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :
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