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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   YI016 Antibody (C-term)   

YI016 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - YI016 Antibody (C-term) AP11654b
    Confocal immunofluorescent analysis of YI016 Antibody (C-term)(Cat#AP11654b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - YI016 Antibody (C-term) AP11654b
    YI016 Antibody (C-term) (Cat. #AP11654b) western blot analysis in K562,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the YI016 antibody detected the YI016 protein (arrow).
    detail
  • IHC-P - YI016 Antibody (C-term) AP11654b
    YI016 Antibody (C-term) (Cat. #AP11654b)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of YI016 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - YI016 Antibody (C-term) AP11654b
    YI016 Antibody (C-term) (Cat. #AP11654b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC, WB, IHC-P, E
Primary Accession A6NKZ8
Other Accession A6NNZ2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 253-280 aa
Additional Information
Other Names Putative tubulin beta chain-like protein ENSP00000290377
Target/Specificity This YI016 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-280 amino acids from the C-terminal region of human YI016.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsYI016 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain (By similarity).

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$ 365.00
$ 140.00
Cat# AP11654b
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