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UQCRFS1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - UQCRFS1 Antibody (C-term) AP11659b
    Anti-UQCRFS1 Antibody (C-term) at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - UQCRFS1 Antibody (C-term) AP11659b
    UQCRFS1 Antibody (C-term) (Cat. #AP11659b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the UQCRFS1 antibody detected the UQCRFS1 protein (arrow).
  • IHC-P - UQCRFS1 Antibody (C-term) AP11659b
    UQCRFS1 Antibody (C-term) (Cat. #AP11659b)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UQCRFS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - UQCRFS1 Antibody (C-term) AP11659b
    UQCRFS1 Antibody (C-term) (Cat. #AP11659b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - UQCRFS1 Antibody (C-term) AP11659b
    Confocal immunofluorescent analysis of UQCRFS1 Antibody (C-term)(Cat#AP11659b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P47985
Other Accession Q9CR68, Q5ZLR5, P0C7P4, NP_005994.2
Reactivity Human
Predicted Chicken, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 29668 Da
Antigen Region 181-209 aa
Additional Information
Gene ID 7386
Other Names Cytochrome b-c1 complex subunit Rieske, mitochondrial, Complex III subunit 5, Cytochrome b-c1 complex subunit 5, Rieske iron-sulfur protein, RISP, Ubiquinol-cytochrome c reductase iron-sulfur subunit, Cytochrome b-c1 complex subunit 11, Complex III subunit IX, Ubiquinol-cytochrome c reductase 8 kDa protein, UQCRFS1
Target/Specificity This UQCRFS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-209 amino acids from the C-terminal region of human UQCRFS1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUQCRFS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein
Research Areas
Citations (0)

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Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex (By similarity).


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Grimwood, J., et al. Nature 428(6982):529-535(2004)
Ohashi, Y., et al. Gynecol. Oncol. 93(1):54-58(2004)

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$ 295.00
$ 99.00
Cat# AP11659b
(40 western blots)
Availability: In Stock
Bulk Size
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