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PTK9L Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PTK9L Antibody (N-term) AP11665a
    Western blot analysis of PTK9L (arrow) using rabbit polyclonal PTK9L Antibody (N-term) (Cat. #AP11665a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PTK9L gene.
  • WB - PTK9L Antibody (N-term) AP11665a
    PTK9L Antibody (N-term) (Cat. #AP11665a) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PTK9L antibody detected the PTK9L protein (arrow).
  • IHC-P - PTK9L Antibody (N-term) AP11665a
    PTK9L Antibody (N-term) (Cat. #AP11665a)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTK9L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PTK9L Antibody (N-term) AP11665a
    PTK9L Antibody (N-term) (Cat. #AP11665a) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6IBS0
Other Accession Q9Z0P5, NP_009215.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39548 Da
Antigen Region 28-57 aa
Additional Information
Gene ID 11344
Other Names Twinfilin-2, A6-related protein, hA6RP, Protein tyrosine kinase 9-like, Twinfilin-1-like protein, TWF2, PTK9L
Target/Specificity This PTK9L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human PTK9L.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTK9L Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TWF2
Synonyms PTK9L
Function Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia (By similarity).
Cellular Location Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Cell projection, stereocilium. Note=Perinuclear and G-actin-rich cortical actin structure sublocalization
Tissue Location Ubiquitously expressed (at protein level).
Research Areas
Citations (0)

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The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)

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Cat# AP11665a
(40 western blots)
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