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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   BTC Antibody (N-term)   

BTC Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BTC Antibody (N-term) AP11669a
    BTC Antibody (N-term) (Cat. #AP11669a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the BTC antibody detected the BTC protein (arrow).
  • IHC-P - BTC Antibody (N-term) AP11669a
    BTC Antibody (N-term) (Cat. #AP11669a)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - BTC Antibody (N-term) AP11669a
    Confocal immunofluorescent analysis of BTC Antibody (N-term)(Cat#AP11669a) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - BTC Antibody (N-term) AP11669a
    BTC Antibody (N-term) (Cat. #AP11669a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P35070
Other Accession NP_001720.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 17-45 aa
Additional Information
Other Names Probetacellulin, Betacellulin, BTC, BTC
Target/Specificity This BTC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human BTC.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBTC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BTC
Function Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.
Cellular Location Betacellulin: Secreted, extracellular space.
Tissue Location Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine EMBL; S55606; AAB25452.1; -; mRNA EMBL; BC011618; AAH11618.1; -; mRNA CCDS; CCDS3566.1; - PIR; JC1467; JC1467 RefSeq; NP_001303892.1; NM_001316963.1 RefSeq; NP_001720.1; NM_001729.3 RefSeq; XP_011530513.1; XM_011532211.1 UniGene; Hs.591704; - UniGene; Hs.710156; - PDB; 1IOX; NMR; -; A=62-111 PDB; 1IP0; NMR; -; A=62-111 PDBsum; 1IOX; - PDBsum; 1IP0; - ProteinModelPortal; P35070; - SMR; P35070; - BioGrid; 107150; 20 DIP; DIP-5768N; - IntAct; P35070; 7 MINT; P35070; - STRING; 9606.ENSP00000379092; - PhosphoSitePlus; P35070; - SwissPalm; P35070; - BioMuta; BTC; - DMDM; 461653; - PaxDb; P35070; - PeptideAtlas; P35070; - PRIDE; P35070; - ProteomicsDB; 54977; - DNASU; 685; - Ensembl; ENST00000395743; ENSP00000379092; ENSG00000174808 GeneID; 685; - KEGG; hsa:685; - UCSC; uc003hig.3; human CTD; 685; - DisGeNET; 685; - EuPathDB; HostDB:ENSG00000174808.11; - GeneCards; BTC; - HGNC; HGNC:1121; BTC MIM; 600345; gene neXtProt; NX_P35070; - OpenTargets; ENSG00000174808; - PharmGKB; PA25442; - eggNOG; ENOG410IWUI; Eukaryota eggNOG; ENOG41126JP; LUCA GeneTree; ENSGT00730000110951; - HOGENOM; HOG000237352; - HOVERGEN; HBG004905; - InParanoid; P35070; - KO; K09783; - OMA; ILHCVVA; - OrthoDB; EOG091G0RK4; - PhylomeDB; P35070; - TreeFam; TF332938; - Reactome; R-HSA-1227986; Signaling by ERBB2 Reactome; R-HSA-1236394; Signaling by ERBB4 Reactome; R-HSA-1250196; SHC1 events in ERBB2 signaling Reactome; R-HSA-1250342; PI3K events in ERBB4 signaling Reactome; R-HSA-1250347; SHC1 events in ERBB4 signaling Reactome; R-HSA-1251985; Nuclear signaling by ERBB4 Reactome; R-HSA-1257604; PIP3 activates AKT signaling Reactome; R-HSA-1963640; GRB2 events in ERBB2 signaling Reactome; R-HSA-1963642; PI3K events in ERBB2 signaling Reactome; R-HSA-2219530; Constitutive Signaling by Aberrant PI3K in Cancer Reactome; R-HSA-5673001; RAF/MAP kinase cascade Reactome; R-HSA-6785631; ERBB2 Regulates Cell Motility Reactome; R-HSA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling Reactome; R-HSA-8847993; ERBB2 Activates PTK6 Signaling Reactome; R-HSA-8863795; Downregulation of ERBB2 signaling SignaLink; P35070; - SIGNOR; P35070; - ChiTaRS; BTC; human EvolutionaryTrace; P35070; - GenomeRNAi; 685; - PRO; PR:P35070; - Proteomes; UP000005640; Chromosome 4 Bgee; ENSG00000174808; - CleanEx; HS_BTC; - ExpressionAtlas; P35070; baseline and differential Genevisible; P35070; HS GO; GO:0005576; C:extracellular region; TAS:Reactome GO; GO:0005615; C:extracellular space; IBA:GO_Central GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW GO; GO:0005622; C:intracellular; IEA:GOC GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell GO; GO:0005154; F:epidermal growth factor receptor binding; IBA:GO_Central GO; GO:0008083; F:growth factor activity; IBA:GO_Central GO; GO:0046934; F:phosphatidylinositol-4,5-bisphosphate 3-kinase activity; TAS:Reactome GO; GO:0004713; F:protein tyrosine kinase activity; TAS:Reactome GO; GO:0005088; F:Ras guanyl-nucleotide exchange factor activity; TAS:Reactome GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IBA:GO_Central GO; GO:0038128; P:ERBB2 signaling pathway; TAS:Reactome GO; GO:0000165; P:MAPK cascade; TAS:Reactome GO; GO:0043066; P:negative regulation of apoptotic process; IEA:Ensembl GO; GO:0045597; P:positive regulation of cell differentiation; IEA:Ensembl GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW GO; GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc GO; GO:0048146; P:positive regulation of fibroblast proliferation; IEA:Ensembl GO; GO:0045840; P:positive regulation of mitotic nuclear division; IEA:Ensembl GO; GO:0051897; P:positive regulation of protein kinase B signaling; TAS:Reactome GO; GO:0035810; P:positive regulation of urine volume; IEA:Ensembl GO; GO:2000145; P:regulation of cell motility; TAS:Reactome InterPro; IPR013032; EGF-like_CS InterPro; IPR000742; EGF-like_dom InterPro; IPR015497; EGF_rcpt_ligand PANTHER; PTHR10740; PTHR10740; 1 PROSITE; PS00022; EGF_1; 1 PROSITE; PS01186; EGF_2; 1 PROSITE; PS50026; EGF_3; 1 1: Evidence at protein level; 3D-structure; Cell membrane; Complete proteome; Disulfide bond; EGF-like domain; Glycoprotein; Growth factor; Membrane; Mitogen; Polymorphism; Reference proteome; Secreted; Signal; Transmembrane; Transmembrane helix SIGNAL 1 31 CHAIN 32 178 Probetacellulin /FTId=PRO_0000300685 CHAIN 32 111 Betacellulin /FTId=PRO_0000007490 PROPEP 112 178 Removed in mature form. /FTId=PRO_0000007491 TOPO_DOM 32 118 Extracellular. TRANSMEM 119 139 Helical. TOPO_DOM 140 178 Cytoplasmic. DOMAIN 65 105 EGF-like. {ECO:0000255|PROSITE- ProRule:PRU00076} COMPBIAS 146 154 Arg/Lys-rich (basic) CARBOHYD 34 34 N-linked (GlcNAc...) asparagine DISULFID 69 82 DISULFID 77 93 DISULFID 95 104 VARIANT 7 7 C -> G (in dbSNP:rs28549760) /FTId=VAR_029307 VARIANT 44 44 L -> F (in dbSNP:rs56320257) /FTId=VAR_061151 VARIANT 124 124 L -> M (in dbSNP:rs11938093) /FTId=VAR_029308 STRAND 66 68 {ECO:0000244|PDB:1IP0} HELIX 71 73 {ECO:0000244|PDB:1IOX} STRAND 82 85 {ECO:0000244|PDB:1IOX} TURN 86 89 {ECO:0000244|PDB:1IOX} STRAND 90 93 {ECO:0000244|PDB:1IOX} TURN 101 104 {ECO:0000244|PDB:1IOX} STRAND 106 108 {ECO:0000244|PDB:1IP0} SEQUENCE 178 AA; 19746 MW; 27AC77BD92001F0F CRC64; MDRAARCSGA SSLPLLLALA LGLVILHCVV ADGNSTRSPE TNGLLCGDPE ENCAATTTQS KRKGHFSRCP KQYKHYCIKG RCRFVVAEQT PSCVCDEGYI GARCERVDLF YLRGDRGQIL VICLIAVMVV FIILVIGVCT CCHPLRKRRK RKKKEEEMET LGKDITPINE DIEETNIA
Research Areas
Citations (0)

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Background

The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.

References

Stoeck, A., et al. J. Cell. Sci. 123 (PT 13), 2319-2331 (2010) :
Genetos, D.C., et al. Cell Tissue Res. 340(1):81-89(2010)
Nagaoka, T., et al. J. Mol. Biol. 380(1):83-94(2008)
Revillion, F., et al. Ann. Oncol. 19(1):73-80(2008)
Moss, M.L., et al. J. Biol. Chem. 282(49):35712-35721(2007)

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Cat# AP11669a
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