|Application ||WB, IF, E|
|Other Accession||P20171, Q61411, P08642, NP_005334.1|
|Calculated MW||21298 Da|
|Antigen Region||146-176 aa|
|Other Names||GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1|
|Target/Specificity||This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-176 amino acids from the C-terminal region of human HRAS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HRAS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).|
|Cellular Location||Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
Ma, Z., et al. Oncogene 29(41):5559-5567(2010)
van Engen-van Grunsven, A.C., et al. Am. J. Surg. Pathol. 34(10):1436-1441(2010)
Li, H., et al. Oncogene 29(36):5083-5094(2010)
Kwack, K.B., et al. Korean J Gastroenterol 56(2):78-82(2010)
Amosenko, F.A., et al. Genetika 46(5):700-708(2010)
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