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HRAS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - HRAS Antibody (C-term) AP11676b
    Confocal immunofluorescent analysis of HRAS Antibody (C-term)(Cat#AP11676b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
    detail
  • WB - HRAS Antibody (C-term) AP11676b
    Western blot analysis of HRAS (arrow) using rabbit polyclonal HRAS Antibody (C-term) (Cat. #AP11676b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HRAS gene.
    detail
  • WB - HRAS Antibody (C-term) AP11676b
    HRAS Antibody (C-term) (Cat. #AP11676b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the HRAS antibody detected the HRAS protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P01112
Other Accession P20171, Q61411, P08642, NP_005334.1
Reactivity Human, Mouse
Predicted Chicken, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 21298 Da
Antigen Region 146-176 aa
Additional Information
Gene ID 3265
Other Names GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1
Target/Specificity This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-176 amino acids from the C-terminal region of human HRAS.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHRAS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HRAS
Synonyms HRAS1
Function Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor. Note=The active GTP-bound form is localized most strongly to membranes than the inactive GDP-bound form (By similarity). Shuttles between the plasma membrane and the Golgi apparatus.
Tissue Location Widely expressed..
Research Areas
Citations (0)
citation

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Background

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

References

Ma, Z., et al. Oncogene 29(41):5559-5567(2010)
van Engen-van Grunsven, A.C., et al. Am. J. Surg. Pathol. 34(10):1436-1441(2010)
Li, H., et al. Oncogene 29(36):5083-5094(2010)
Kwack, K.B., et al. Korean J Gastroenterol 56(2):78-82(2010)
Amosenko, F.A., et al. Genetika 46(5):700-708(2010)

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$ 182.50
$ 70.00
Cat# AP11676b
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