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LSM7 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - LSM7 Antibody (C-term) AP11679b
    LSM7 Antibody (C-term) (Cat. #AP11679b) western blot analysis in CEM cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the LSM7 antibody detected the LSM7 protein (arrow).
    detail
  • WB - LSM7 Antibody (C-term) AP11679b
    Anti-LSM7 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9UK45
Other Accession Q9CQQ8, NP_057283.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 11602 Da
Antigen Region 45-72 aa
Additional Information
Gene ID 51690
Other Names U6 snRNA-associated Sm-like protein LSm7, LSM7
Target/Specificity This LSM7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-72 amino acids from the C-terminal region of human LSM7.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLSM7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LSM7
Function Plays a role in pre-mRNA splicing as component of the U4/U6- U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (PubMed:28781166). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA (PubMed:10523320).
Cellular Location Nucleus
Research Areas
Citations (0)
citation

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Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family (see SNRPD2; MIM 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

References

Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Grimwood, J., et al. Nature 428(6982):529-535(2004)
Lehner, B., et al. Genomics 83(1):153-167(2004)
Ingelfinger, D., et al. RNA 8(12):1489-1501(2002)
Conte, N., et al. Oncogene 21(36):5619-5630(2002)

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$ 365.00
$ 140.00
Cat# AP11679b
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