|Application ||WB, FC, E|
|Calculated MW||131295 Da|
|Antigen Region||546-574 aa|
|Other Names||Partner and localizer of BRCA2, PALB2, FANCN|
|Target/Specificity||This PALB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 546-574 amino acids from the Central region of human PALB2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PALB2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity.|
|Cellular Location||Nucleus. Note=Colocalizes with BRCA2 in nuclear foci|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a protein that may function in tumor suppression. This protein binds to and colocalizes with the breast cancer 2 early onset protein (BRCA2) in nuclear foci and likely permits the stable intranuclear localization and accumulation of BRCA2.
Dray, E., et al. Nat. Struct. Mol. Biol. 17(10):1255-1259(2010)
Buisson, R., et al. Nat. Struct. Mol. Biol. 17(10):1247-1254(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Adank, M.A., et al. Pediatr Blood Cancer 55(4):742-744(2010)
Guenard, F., et al. Genet Test Mol Biomarkers 14(4):515-526(2010)
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