|Application ||WB, FC, E|
|Calculated MW||13157 Da|
|Antigen Region||34-60 aa|
|Other Names||Melanoma antigen recognized by T-cells 1, MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, MLANA, MART1|
|Target/Specificity||This MART-1/Melan-A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-60 amino acids from the Central region of human MART-1/Melan-A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MART-1/Melan-A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type III membrane protein. Golgi apparatus. Golgi apparatus, trans-Golgi network membrane. Melanosome. Note=Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation|
|Tissue Location||Expression is restricted to melanoma and melanocyte cell lines and retina|
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Provided below are standard protocols that you may find useful for product applications.
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein SILV/PMEL17, which is critical to the formation of stage II melanosomes.
Li, Y., et al. J. Mol. Biol. 399(4):596-603(2010)
Giordano, F., et al. Hum. Mol. Genet. 18(23):4530-4545(2009)
Fernandez, L.P., et al. Exp. Dermatol. 18(7):634-642(2009)
Beltraminelli, H., et al. Am J Dermatopathol 31(3):305-308(2009)
Serana, F., et al. J Transl Med 7, 21 (2009) :
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