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PAP Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PAP Antibody (Center) AP11691c
    PAP Antibody (Center) (Cat. #AP11691c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PAP antibody detected the PAP protein (arrow).
  • IHC-P - PAP Antibody (Center) AP11691c
    PAP Antibody (Center) (Cat. #AP11691c)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P51003
Other Accession P25500, NP_116021.2
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 82843 Da
Antigen Region 407-435 aa
Additional Information
Gene ID 10914
Other Names Poly(A) polymerase alpha, PAP-alpha, Polynucleotide adenylyltransferase alpha, PAPOLA, PAP
Target/Specificity This PAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the Central region of human PAP.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAPOLA
Synonyms PAP
Function Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
Cellular Location Cytoplasm. Nucleus. Note=The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic
Citations (0)

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Background

Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.

References

Rapti, A., et al. Mol. Cell. Biochem. 340 (1-2), 91-96 (2010) :
Nan, H., et al. J. Invest. Dermatol. 129(9):2250-2257(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Dettwiler, S., et al. J. Biol. Chem. 279(34):35788-35797(2004)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)

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Cat# AP11691c
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