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PAX5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PAX5 Antibody (Center) AP11704C
    PAX5 Antibody (Center) (Cat. #AP11704c) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PAX5 antibody detected the PAX5 protein (arrow).
  • FC - PAX5 Antibody (Center) AP11704C
    PAX5 Antibody (Center) (Cat. #AP11704c) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - PAX5 Antibody (Center) AP11704C
    PAX5 Antibody (Center) (Cat. #AP11704c)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q02548
Other Accession Q02650, NP_057953.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42149 Da
Antigen Region 178-207 aa
Additional Information
Gene ID 5079
Other Names Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5
Target/Specificity This PAX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the Central region of human PAX5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAX5
Function May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Cellular Location Nucleus.
Research Areas
Citations (0)

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This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.


Song, J., et al. Arch. Pathol. Lab. Med. 134(11):1702-1705(2010)
Okamoto, R., et al. Haematologica 95(9):1481-1488(2010)
Best, A., et al. Leuk. Res. 34(8):1098-1102(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Vidal, L.J., et al. Mol. Cancer Res. 8(3):444-456(2010)

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$ 295.00
$ 99.00
Cat# AP11704C
(40 western blots)
Availability: In Stock
Bulk Size
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