|Application ||WB, FC, E|
|Other Accession||NP_563578.1, NP_002829.2|
|Calculated MW||147254 Da|
|Antigen Region||336-364 aa|
|Other Names||Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45|
|Target/Specificity||This PTPRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the N-terminal region of human PTPRC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PTPRC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein Membrane raft. Note=Colocalized with DPP4 in membrane rafts|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq].
Heyd, F., et al. Mol. Cell 40(1):126-137(2010)
Wu, Z., et al. J. Immunol. 185(1):231-238(2010)
Cui, J., et al. Arthritis Rheum. 62(7):1849-1861(2010)
Booth, N.J., et al. J. Immunol. 184(8):4317-4326(2010)
Capitanescu, B., et al. Rom J Morphol Embryol 51(1):49-54(2010)
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