|Application ||IHC-P, WB, E|
|Other Accession||Q7TN33, NP_001166155.1, NP_443072.3|
|Calculated MW||50477 Da|
|Antigen Region||46-74 aa|
|Other Names||CUGBP Elav-like family member 6, CELF-6, Bruno-like protein 6, CUG-BP- and ETR-3-like factor 6, RNA-binding protein BRUNOL-6, CELF6, BRUNOL6|
|Target/Specificity||This BRUNOL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-74 amino acids from the N-terminal region of human BRUNOL6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BRUNOL6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||RNA-binding protein implicated in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in a muscle-specific splicing enhancer (MSE)-dependent manner. Promotes also exon exclusion of INSR pre-mRNA.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Expressed mainly in kidney, brain and testis and present in other tissues albeit at lower levels. Also expressed in fetal kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Ladd, A.N., et al. J. Biol. Chem. 279(17):17756-17764(2004)
Wistow, G., et al. Mol. Vis. 8, 205-220 (2002) :
Good, P.J., et al. J. Biol. Chem. 275(37):28583-28592(2000)
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