- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
SMAD3 Antibody (S213)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Alternative | |||
| AP11730a | AP13540c, AP6267b, AP9995a | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | ||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
SMAD3 Antibody (S213) - Product info | |
| Application | WB, IHC, IF, FC
|
| Primary Accession | P84022 |
| Other Accession | P84025, P84024, Q8BUN5, P84023, NP_001138575.1, NP_005893.1 |
| Reactivity | Human |
| Predicted | Chicken, Mouse, Pig, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 48081 Da |
SMAD3 Antibody (S213) - Additional info | |
| Gene ID 4088 | |
| Other Names SMAD3; MADH3; Mothers against decapentaplegic homolog 3; JV15-2; SMAD family member 3 | |
| Target/Specificity This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the human SMAD3. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SMAD3 Antibody (S213) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SMAD3 Antibody (S213) - Protein Information | |
| Name SMAD3 | |
| Synonyms MADH3 | |
| Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator | |
| Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta | |
SMAD3 Antibody (S213) - Related products
AP11730a: SMAD3 Antibody (S213)
AP13540c: SMAD3 Antibody (Center)
AP3249a: Phospho-SMAD3-S208 Antibody
AP3250a: Phospho-SMAD3-S213 Antibody
AP6267a: SMAD3 Antibody (Center D201)
AP6267b: SMAD3 Antibody (Center N206)
RI14954: SMAD3 predesign siRNA
LY12944a: SMAD3 Over-expression Lysate
BP3248a: Phospho-SMAD3-S204 Antibody Blocking Peptide
BP3249a: Phospho-SMAD3-S208 Antibody Blocking Peptide
BP3250a: Phospho-SMAD3-S213 Antibody Blocking Peptide
BP6267a: SMAD3 Antibody (Center D201) Blocking Peptide
BP6267b: SMAD3 Antibody (Center N206) Blocking Peptide
BP9995a: SMAD3-S208 Antibody Blocking Peptide
SMAD3 Antibody (S213) - Application data
-
SMAD3 Antibody (S213) (Cat. #AP11730a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).
-
SMAD3 Antibody (S213) (Cat. #AP11730a)immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMAD3 Antibody (S213) for immunohistochemistry. Clinical relevance has not been evaluated.
-
Confocal immunofluorescent analysis of SMAD3 Antibody (S213)(Cat#AP11730a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
-
SMAD3 Antibody (S213) (Cat. #AP11730a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Confocal immunofluorescent analysis of SMAD3 Antibody (S213)(Cat#AP11730a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
SMAD3 Antibody (S213) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
REFERENCES
Ge, Q., et al. J. Cell. Physiol. 225(3):846-854(2010) Lee, J., et al. J. Biol. Chem. 285(34):26618-26627(2010) Roder, C., et al. Childs Nerv Syst (2010) In press : Valdes, A.M., et al. Arthritis Rheum. 62(8):2347-2352(2010) Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
