|Application ||WB, E|
|Other Accession||NP_149083.2, NP_149084.2, NP_149023.2|
|Calculated MW||56338 Da|
|Antigen Region||371-399 aa|
|Other Names||Tripartite motif-containing protein 5, 632-, RING finger protein 88, TRIM5, RNF88|
|Target/Specificity||This TRIM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-399 amino acids from the C-terminal region of human TRIM5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TRIM5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF- kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV) and equine infectious anemia virus (EIAV).|
|Cellular Location||Cytoplasm, P-body Note=Closely associates with proteasomal subunits in cytoplasmic bodies (By similarity). Colocalizes with SQSTM1 in cytoplasmic bodies.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Ohmine, S., et al. J. Biol. Chem. 285(45):34508-34517(2010)
Battivelli, E., et al. J. Virol. 84(21):11010-11019(2010)
Malbec, M., et al. Virology 405(2):414-423(2010)
Price, H., et al. AIDS 24(12):1813-1821(2010)
Kuroishi, A., et al. Retrovirology 7, 58 (2010) :
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