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RHOJ Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RHOJ Antibody (Center) AP11763c
    RHOJ Antibody (Center) (Cat. #AP11763c) western blot analysis in HepG2,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RHOJ antibody detected the RHOJ protein (arrow).
    detail
  • IHC-P - RHOJ Antibody (Center) AP11763c
    RHOJ Antibody (Center) (Cat. #AP11763c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RHOJ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9H4E5
Other Accession Q9ER71, NP_065714.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 23821 Da
Antigen Region 131-159 aa
Additional Information
Gene ID 57381
Other Names Rho-related GTP-binding protein RhoJ, Ras-like protein family member 7B, Tc10-like GTP-binding protein, RHOJ, ARHJ, RASL7B, RHOI, TCL
Target/Specificity This RHOJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the Central region of human RHOJ.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRHOJ Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RHOJ
Function Plasma membrane-associated small GTPase specifically involved in angiogenesis (PubMed:21628409, PubMed:24434213, PubMed:30158707). Required for endothelial cell migration during vascular development via its interaction with GLUL (PubMed:30158707). Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration (PubMed:30158707).
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side Note=Localization to the plasma membrane is regulated by GLUL
Tissue Location Specifically expressed in endothelial cells in different tissues, such as brain, heart, lung and liver
Research Areas
Citations (0)
citation

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Background

ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
Nishikimi, A., et al. FEBS Lett. 579(5):1039-1046(2005)
Ruusala, A., et al. FEBS Lett. 572 (1-3), 159-166 (2004) :

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$ 365.00
$ 140.00
Cat# AP11763c
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