|Application ||WB, E|
|Other Accession||NP_620278.1, NP_004597.2|
|Calculated MW||212677 Da|
|Antigen Region||1863-1893 aa|
|Other Names||Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, p250, Transcription initiation factor TFIID 250 kDa subunit, TAF(II)250, TAFII-250, TAFII250, TAF1, BA2R, CCG1, CCGS, TAF2A|
|Target/Specificity||This TAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1863-1893 amino acids from the C-terminal region of human TAF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TAF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BA2R, CCG1, CCGS, TAF2A|
|Function||Largest component and core scaffold of the TFIID basal transcription factor complex (PubMed:25412659, PubMed:27007846). Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2- mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity (PubMed:25412659). Essential for progression of the G1 phase of the cell cycle (PubMed:11278496, PubMed:15053879, PubMed:2038334, PubMed:8450888, PubMed:8625415, PubMed:9660973, PubMed:9858607). Exhibits histone acetyltransferase activity towards histones H3 and H4 (PubMed:15870300).|
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Provided below are standard protocols that you may find useful for product applications.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. This gene is part of a complex transcriptional unit (TAF1/DYT3), wherein some products share exons with TAF1 as well as additional exons downstream.
Tavassoli, P., et al. Mol. Endocrinol. 24(4):696-708(2010)
Allende-Vega, N., et al. Mol. Cell. Biochem. 316 (1-2), 99-106 (2008) :
Centeno, F., et al. Intervirology 51(2):137-143(2008)
Olejnik-Schmidt, A.K., et al. Arch. Virol. 153(5):983-990(2008)
Li, A.G., et al. Mol. Cell 28(3):408-421(2007)
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