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RHEB Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q15382 |
| Other Accession | Q62639, Q921J2, Q56JV3, NP_005605.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20497 Da |
| Antigen Region | 104-134 aa |
| Gene ID | 6009 |
|---|---|
| Other Names | GTP-binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2 |
| Target/Specificity | This RHEB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-134 amino acids from the C-terminal region of human RHEB. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RHEB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RHEB {ECO:0000303|PubMed:8543055, ECO:0000312|HGNC:HGNC:10011} |
|---|---|
| Function | Small GTPase that acts as an allosteric activator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:12172553, PubMed:12906785, PubMed:12271141, PubMed:12842888, PubMed:12869586, PubMed:15340059, PubMed:15854902, PubMed:16098514, PubMed:20381137, PubMed:24529379, PubMed:22819219, PubMed:29416044, PubMed:32470140, PubMed:33157014). In response to nutrients, growth factors or amino acids, specifically activates the protein kinase activity of MTOR, the catalytic component of the mTORC1 complex: acts by causing a conformational change that allows the alignment of residues in the active site of MTOR, thereby enhancing the phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) (PubMed:33157014, PubMed:29236692). RHEB is also required for localization of the TSC-TBC complex to lysosomal membranes (PubMed:24529379). In response to starvation, RHEB is inactivated by the TSC-TBC complex, preventing activation of mTORC1 (PubMed:24529379, PubMed:33157014). Has low intrinsic GTPase activity (PubMed:15340059). |
| Cellular Location | Endomembrane system; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid- anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. Note=Farnesylation is required for recruitment to lysosomal membranes, where it activates the mTORC1 complex. |
| Tissue Location | Ubiquitous (PubMed:8543055). Highest levels observed in skeletal and cardiac muscle (PubMed:8543055) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
References
Zheng, H., et al. Cancer Lett. 297(1):117-125(2010)
Kim, H.W., et al. Mol. Cell. Biol. 30(22):5406-5420(2010)
Karassek, S., et al. J. Biol. Chem. 285(44):33979-33991(2010)
Wagner, R.J., et al. Am. J. Physiol., Cell Physiol. 299 (1), C119-C127 (2010) :
Lu, Z.H., et al. Cancer Res. 70(8):3287-3298(2010)
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