|Application ||IF, WB, IHC-P, FC, E|
|Calculated MW||131117 Da|
|Antigen Region||830-860 aa|
|Other Names||Nitric oxide synthase, inducible, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, NOS2A|
|Target/Specificity||This NOS2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 830-860 amino acids from the Central region of human NOS2A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NOS2A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. As component of the iNOS- S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys- 247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM.|
|Tissue Location||Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Ryk, C., et al. J. Urol. 184(5):2150-2157(2010)
Planche, T., et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 299 (5), R1248-R1253 (2010) :
Feng, C., et al. FEBS Lett. 584(20):4335-4338(2010)
Mokrzycka, M., et al. Folia Histochem. Cytobiol. 48(2):191-196(2010)
Tupitsyna, T.V., et al. Mol. Gen. Mikrobiol. Virusol. 3, 3-7 (2010) :
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.