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NBN Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NBN Antibody (C-term) AP11776b
    NBN Antibody (C-term) (Cat. #AP11776b) western blot analysis in NCI-H460,NCI-H292 cell line lysates (35ug/lane).This demonstrates the NBN antibody detected the NBN protein (arrow).
  • IF - NBN Antibody (C-term) AP11776b
    Confocal immunofluorescent analysis of NBN Antibody (C-term) (Cat. #AP11776b) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - NBN Antibody (C-term) AP11776b
    NBN Antibody (C-term) (Cat. #AP11776b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60934
Other Accession NP_002476.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 84959 Da
Antigen Region 602-630 aa
Additional Information
Gene ID 4683
Other Names Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, NBN, NBS, NBS1, P95
Target/Specificity This NBN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 602-630 amino acids from the C-terminal region of human NBN.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNBN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NBN
Synonyms NBS, NBS1, P95
Function Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
Cellular Location Nucleus. Nucleus, PML body Chromosome, telomere. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents.
Tissue Location Ubiquitous. Expressed at high levels in testis
Research Areas
Citations (0)

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Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.


Liu, Y., et al. Carcinogenesis 31(10):1762-1769(2010)
Kavitha, C.V., et al. Biochem. Biophys. Res. Commun. 399(4):575-580(2010)
Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
Jelonek, K., et al. J. Appl. Genet. 51(3):343-352(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :

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Cat# AP11776b
(40 western blots)
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