|Application ||WB, IHC-P, IF, FC, E|
|Calculated MW||50688 Da|
|Antigen Region||376-404 aa|
|Other Names||Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin I, Vasostatin-2, Vasostatin II, EA-92, ES-43, Pancreastatin, SS-18, WA-8, WE-14, LF-19, AL-11, GV-19, GR-44, ER-37, CHGA|
|Target/Specificity||This CMGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-404 amino acids from the C-terminal region of human CMGA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CMGA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.|
|Cellular Location||Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.|
|Tissue Location||GE-25 is found in the brain.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
Ezzi, S.A., et al. J. Neurochem. 115(5):1102-1111(2010)
Ma, Z., et al. J. Urol. 184(3):1182-1188(2010)
Ramella, R., et al. J. Cell. Biochem. 110(1):70-79(2010)
Dag, E., et al. Peptides 31(5):932-937(2010)
Xie, Y.Q., et al. Zhonghua Xin Xue Guan Bing Za Zhi 37(12):1081-1084(2009)
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