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AGBL5 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P, WB, E |
|---|---|
| Primary Accession | Q8NDL9 |
| Other Accession | Q58CX9, NP_068603.4 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 97534 Da |
| Antigen Region | 99-127 aa |
| Gene ID | 60509 |
|---|---|
| Other Names | Cytosolic carboxypeptidase-like protein 5, 3417-, ATP/GTP-binding protein-like 5, AGBL5, CCP5 |
| Target/Specificity | This AGBL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the N-terminal region of human AGBL5. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | AGBL5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AGBL5 (HGNC:26147) |
|---|---|
| Function | Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta- tubulin. Cleaves alpha- and gamma-linked polyglutamate tubulin side- chain, as well as the branching point glutamate. Also catalyzes the removal of alpha-linked glutamate residues from the carboxy-terminus of alpha-tubulin. Mediates deglutamylation of nucleotidyltransferase CGAS, leading to CGAS antiviral defense response activation. |
| Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q09M02}. Nucleus Cytoplasm, cytoskeleton, spindle. Midbody. Note=Mainly cytoplasmic. Slight accumulation in the nucleus is observed (By similarity). Colocalizes with alpha-tubulin in the mitotic spindle and with midbody microtubules in the intercellular bridges formed during cytokinesis {ECO:0000250|UniProtKB:Q09M02, ECO:0000269|PubMed:23085998} |
| Tissue Location | Expressed in brain. |
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Provided below are standard protocols that you may find useful for product applications.
Background
AGBL5 has a function in the processing of cytosolic proteins such as alpha tubulin, which is known to be modified by the removal of a C terminal tyrosine. It is expressed in the brain. There are three named isoforms.
References
Bailey, S.D., et al. Diabetes Care (2010) In press :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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