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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IER3 Antibody (N-term)   

IER3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IER3 Antibody (N-term) AP11790a
    IER3 Antibody (N-term) (Cat. #AP11790a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).
  • WB - IER3 Antibody (N-term) AP11790a
    IER3 Antibody (N-term) (Cat. #AP11790a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IER3 antibody detected the IER3 protein (arrow).
  • IHC-P - IER3 Antibody (N-term) AP11790a
    IER3 Antibody (N-term) (Cat. #AP11790a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IER3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - IER3 Antibody (N-term) AP11790a
    Confocal immunofluorescent analysis of IER3 Antibody (N-term)(Cat#AP11790a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - IER3 Antibody (N-term) AP11790a
    IER3 Antibody (N-term) (Cat. #AP11790a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, FC, E
Primary Accession P46695
Other Accession NP_003888.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16903 Da
Antigen Region 38-65 aa
Additional Information
Gene ID 8870
Other Names Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Protein DIF-2, Immediate early protein GLY96, Immediate early response 3 protein, PACAP-responsive gene 1 protein, Protein PRG1, IER3, DIF2, IEX1, PRG1
Target/Specificity This IER3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 amino acids from the N-terminal region of human IER3.
Dilution WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIER3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IER3
Synonyms DIF2, IEX1, PRG1
Function May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
Cellular Location Membrane; Single-pass type II membrane protein
Research Areas
Citations ( 0 )

Background

This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein.

References

Shahid, M., et al. Hypertension 56(4):705-712(2010)
Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009) :
Do, T.N., et al. Cancer Genet. Cytogenet. 195(1):31-36(2009)
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :

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Cat# AP11790a
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(40 western blots)
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