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DEF Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - DEF Antibody  (N-term) AP11810a
    Overlay histogram showing U-2OS cells stained with AP11810a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP11810a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - DEF Antibody  (N-term) AP11810a
    Anti-DEF Antibody (N-term) at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - DEF Antibody  (N-term) AP11810a
    All lanes : Anti-DEF Antibody (N-term) at 1:2000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: RPMI 8226 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 87 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - DEF Antibody  (N-term) AP11810a
    DEF Antibody (N-term) (Cat. #AP11810a)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DEF Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - DEF Antibody  (N-term) AP11810a
    Confocal immunofluorescent analysis of DEF Antibody (N-term)(Cat#AP11810a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q68CQ4
Other Accession Q5M9G7, Q8BTT6, NP_055203.4
Reactivity Human, Hamster, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 87055 Da
Antigen Region 2-30 aa
Additional Information
Gene ID 27042
Other Names Digestive organ expansion factor homolog, DIEXF, C1orf107, DEF
Target/Specificity This DEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-30 amino acids from the N-terminal region of human DEF.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDEF Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms C1orf107, DEF
Function Regulates the p53 pathway to control the expansion growth of digestive organs.
Cellular Location Nucleus. Nucleus, nucleolus
Research Areas
Citations (0)

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Regulates the p53 pathway to control the expansion growth of digestive organs (By similarity).


Birnbaum, S., et al. Nat. Genet. 41(4):473-477(2009)
Chen, J., et al. Genes Dev. 19(23):2900-2911(2005)

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$ 295.00
$ 99.00
Cat# AP11810a
(40 western blots)
Availability: In Stock
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