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> home > Products > Primary Antibodies > Antibody Collections > Catalog Updated > TRIM65 Antibody (Center)
TRIM65 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- CITATIONS
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- BACKGROUND
Application 
| WB, FC, IF, IHC-P, E |
|---|---|
| Primary Accession | Q6PJ69 |
| Other Accession | NP_775818.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 57353 Da |
| Antigen Region | 327-355 aa |
| Gene ID | 201292 |
|---|---|
| Other Names | Tripartite motif-containing protein 65, TRIM65 |
| Target/Specificity | This TRIM65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-355 amino acids from the Central region of human TRIM65. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TRIM65 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM65 |
|---|---|
| Function | E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation (PubMed:28594402, PubMed:34512673). Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA- mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (PubMed:24778252). This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy (PubMed:31160576). Upon enteroviral infection, promotes 'Lys-63'- mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade (PubMed:28594402). Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs (PubMed:33373584). Plays also a role in limitation of inflammation through different mechanisms. First, promotes 'Lys-48'-mediated ubiquitination of VCAM1 leading to its degradation and limitation of LPS-induced lung inflammation (PubMed:31310649). In addition, negatively regulates inflammasome activation by promoting 'lys48'-linked ubiquitination of NLRP3 which is critical for the inhibition of NLRP3 inflammasome activation in resting macrophages (PubMed:34512673). |
| Cellular Location | Cytoplasm |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
TRIM65 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger.
References
Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
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$ 365.00
$ 140.00
Cat# AP11814c
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