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TRIM65 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TRIM65  Antibody (Center) AP11814c
    TRIM65 Antibody (Center) (Cat. #AP11814c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TRIM65 antibody detected the TRIM65 protein (arrow).
  • IHC-P - TRIM65  Antibody (Center) AP11814c
    TRIM65 Antibody (Center) (Cat. #AP11814c)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM65 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - TRIM65  Antibody (Center) AP11814c
    Confocal immunofluorescent analysis of TRIM65 Antibody (Center)(Cat#AP11814c) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
  • FC - TRIM65  Antibody (Center) AP11814c
    TRIM65 Antibody (Center) (Cat. #AP11814c) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6PJ69
Other Accession NP_775818.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 57353 Da
Antigen Region 327-355 aa
Additional Information
Gene ID 201292
Other Names Tripartite motif-containing protein 65, TRIM65
Target/Specificity This TRIM65 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-355 amino acids from the Central region of human TRIM65.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTRIM65 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TRIM65
Research Areas
Citations (0)

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TRIM65 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger.


Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :

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Cat# AP11814c
(40 western blots)
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