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COL8A2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP11859B 400 µl (40 western blots) In Stock $ 265.00 Add to cart
AP11859B-ev 80 µl (8 western blots) In Stock $ 95.00 Add to cart
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COL8A2 Antibody (C-term) - Product info

ApplicationWB, IHC, IF
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
Primary AccessionP25067
Other AccessionNP_005193.1
ReactivityHuman
IsotypeRabbit Ig
Calculated MW67244 Da

COL8A2 Antibody (C-term) - Additional info

Gene ID 1296
Other Names
COL8A2; Collagen alpha-2(VIII) chain; Endothelial collagen
Target/Specificity
This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2.
Dilution
WB~~1:1000
IHC~~1:10~50
IF~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
COL8A2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

COL8A2 Antibody (C-term) - Protein Information

Name COL8A2
Function
Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis (By similarity).
Cellular Location
Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Location
Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane

COL8A2 Antibody (C-term) - Related products

BP11859b: COL8A2 Antibody (C-term) Blocking Peptides

COL8A2 Antibody (C-term) - Research Areas

Application data

  • WB - COL8A2 Antibody (C-term) AP11859B

    COL8A2 Antibody (C-term) (Cat. #AP11859b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the COL8A2 antibody detected the COL8A2 protein (arrow).

  • IHC - COL8A2 Antibody (C-term) AP11859B

    COL8A2 Antibody (C-term) (Cat. #AP11859b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of COL8A2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

  • IF - COL8A2 Antibody (C-term) AP11859B

    Confocal immunofluorescent analysis of COL8A2 Antibody (C-term)(Cat#AP11859b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

  • WB - COL8A2 Antibody (C-term) AP11859B
  • IHC - COL8A2 Antibody (C-term) AP11859B
  • IF - COL8A2 Antibody (C-term) AP11859B

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BACKGROUND

This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2.

REFERENCES

Hemadevi, B., et al. BMC Ophthalmol 10, 3 (2010) :
Afshari, N.A., et al. Invest. Ophthalmol. Vis. Sci. 50(3):1093-1097(2009)
Liskova, P., et al. Br J Ophthalmol 91(12):1717-1718(2007)
Aldave, A.J., et al. Cornea 26(8):963-965(2007)
Zhang, C., et al. Trans Am Ophthalmol Soc 104, 85-97 (2006) :