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BTRC Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP11871a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP11871a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
BTRC Antibody (N-term) - Product info | |
| Application | WB, IHC, IF
|
| Primary Accession | Q9Y297 |
| Other Accession | Q3ULA2, NP_378663 |
| Reactivity | Human |
| Predicted | Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 68867 Da |
BTRC Antibody (N-term) - Additional info | |
| Gene ID 8945 | |
| Other Names BTRC; BTRCP; FBW1A; FBXW1A; F-box/WD repeat-containing protein 1A; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; pIkappaBalpha-E3 receptor subunit | |
| Target/Specificity This BTRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-60 amino acids from the N-terminal region of human BTRC. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions BTRC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
BTRC Antibody (N-term) - Protein Information | |
| Name BTRC | |
| Synonyms BTRCP, FBW1A, FBXW1A | |
| Function Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys- 21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3 | |
| Cellular Location Cytoplasm. Nucleus (By similarity). | |
| Tissue Location Expressed in epididymis (at protein level). | |
BTRC Antibody (N-term) - Related products
AP10095a: BTRC Antibody (N-term)
AP11871a: BTRC Antibody (N-term)
BTRC Antibody (N-term) - Application data
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BTRC Antibody (N-term) (Cat. #AP11871a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the BTRC antibody detected the BTRC protein (arrow).
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BTRC Antibody (N-term) (Cat. #AP11871a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of BTRC Antibody (N-term)(Cat#AP11871a) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
BTRC Antibody (N-term) - Research Areas
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BACKGROUND
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.
REFERENCES
Popov, N., et al. Nat. Cell Biol. 12(10):973-981(2010) Inuzuka, H., et al. Cancer Cell 18(2):147-159(2010) Guderian, G., et al. J. Cell. Sci. 123 (PT 13), 2163-2169 (2010) : Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Tsai, W.B., et al. PLoS ONE 5 (7), E11171 (2010) :
