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BTRC Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - BTRC Antibody (N-term) AP11871a
    BTRC Antibody (N-term) (Cat. #AP11871a) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the BTRC antibody detected the BTRC protein (arrow).
  • IHC-P - BTRC Antibody (N-term) AP11871a
    BTRC Antibody (N-term) (Cat. #AP11871a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BTRC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - BTRC Antibody (N-term) AP11871a
    Confocal immunofluorescent analysis of BTRC Antibody (N-term)(Cat#AP11871a) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y297
Other Accession Q3ULA2, NP_378663
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 68867 Da
Antigen Region 17-52 aa
Additional Information
Gene ID 8945
Other Names F-box/WD repeat-containing protein 1A, E3RSIkappaB, Epididymis tissue protein Li 2a, F-box and WD repeats protein beta-TrCP, pIkappaBalpha-E3 receptor subunit, BTRC, BTRCP, FBW1A, FBXW1A
Target/Specificity This BTRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-52 amino acids from the N-terminal region of human BTRC.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBTRC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys- 21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
Cellular Location Cytoplasm. Nucleus.
Tissue Location Expressed in epididymis (at protein level).
Research Areas
Citations ( 0 )


This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.


Popov, N., et al. Nat. Cell Biol. 12(10):973-981(2010) Inuzuka, H., et al. Cancer Cell 18(2):147-159(2010) Guderian, G., et al. J. Cell. Sci. 123 (PT 13), 2163-2169 (2010) : Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : Tsai, W.B., et al. PLoS ONE 5 (7), E11171 (2010) :

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$ 295.00
$ 99.00
Cat# AP11871a
(40 western blots)
Availability: In Stock
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