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PABPN1L Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PABPN1L Antibody (Center) AP11914c
    All lanes : Anti-PABPN1L Antibody (Center) at 1:500-1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - PABPN1L Antibody (Center) AP11914c
    PABPN1L Antibody (Center) (Cat. #AP11914c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PABPN1L Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - PABPN1L Antibody (Center) AP11914c
    Confocal immunofluorescent analysis of PABPN1L Antibody (Center)(Cat#AP11914c) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - PABPN1L Antibody (Center) AP11914c
    PABPN1L Antibody (Center) (Cat. #AP11914c) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession A6NDY0
Other Accession B0BNE4, Q5XFR0, Q6TY21, Q804A5, NP_001073956
Reactivity Human
Predicted Xenopus, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 166-194 aa
Additional Information
Other Names Embryonic polyadenylate-binding protein 2, Embryonic poly(A)-binding protein 2, ePABP-2, ePABP2, Embryonic poly(A)-binding protein type II, Poly(A)-binding protein nuclear-like 1, PABPN1L, EPABP2, PABPNL1
Target/Specificity This PABPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-194 amino acids from the Central region of human PABPN1L.
Dilution WB~~1:500-1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPABPN1L Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms EPABP2, PABPNL1
Function Binds the poly(A) tail of mRNA.
Cellular Location Cytoplasm.
Tissue Location Expressed in various adult tissues. EMBL; AC092384; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC130001; AAI30002.1; -; mRNA EMBL; BC130002; AAI30003.1; -; mRNA CCDS; CCDS45547.2; -. [A6NDY0-1] CCDS; CCDS73925.1; -. [A6NDY0-2] RefSeq; NP_001073956.2; NM_001080487.2. [A6NDY0-1] RefSeq; NP_001281257.1; NM_001294328.1. [A6NDY0-2] RefSeq; XP_016878719.1; XM_017023230.1. [A6NDY0-1] UniGene; Hs.730522; - ProteinModelPortal; A6NDY0; - SMR; A6NDY0; - STRING; 9606.ENSP00000408598; - iPTMnet; A6NDY0; - PhosphoSitePlus; A6NDY0; - BioMuta; PABPN1L; - MaxQB; A6NDY0; - PaxDb; A6NDY0; - PRIDE; A6NDY0; - ProteomicsDB; 940; - ProteomicsDB; 941; -. [A6NDY0-2] ProteomicsDB; 942; -. [A6NDY0-3] ProteomicsDB; 943; -. [A6NDY0-4] TopDownProteomics; A6NDY0-3; -. [A6NDY0-3] DNASU; 390748; - Ensembl; ENST00000411789; ENSP00000405259; ENSG00000205022. [A6NDY0-2] Ensembl; ENST00000419291; ENSP00000408598; ENSG00000205022. [A6NDY0-1] GeneID; 390748; - KEGG; hsa:390748; - UCSC; uc002fmi.4; human. [A6NDY0-1] CTD; 390748; - EuPathDB; HostDB:ENSG00000205022.9; - GeneCards; PABPN1L; - H-InvDB; HIX0059621; - HGNC; HGNC:37237; PABPN1L HPA; HPA043415; - neXtProt; NX_A6NDY0; - OpenTargets; ENSG00000205022; - eggNOG; KOG4209; Eukaryota eggNOG; ENOG4111PFV; LUCA GeneTree; ENSGT00390000001517; - HOGENOM; HOG000208465; - HOVERGEN; HBG107480; - InParanoid; A6NDY0; - KO; K14396; - OMA; IKLKLWA; - OrthoDB; EOG091G0PAK; - PhylomeDB; A6NDY0; - TreeFam; TF105907; - GenomeRNAi; 390748; - PRO; PR:A6NDY0; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000205022; - ExpressionAtlas; A6NDY0; baseline and differential GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW Gene3D;; -; 1 InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf InterPro; IPR035979; RBD_domain_sf InterPro; IPR000504; RRM_dom Pfam; PF00076; RRM_1; 1 SMART; SM00360; RRM; 1 SUPFAM; SSF54928; SSF54928; 1 PROSITE; PS50102; RRM; 1 2: Evidence at transcript level; Alternative splicing; Complete proteome; Cytoplasm; Reference proteome; RNA-binding CHAIN 1 278 Embryonic polyadenylate-binding protein 2 /FTId=PRO_0000349172 DOMAIN 147 224 RRM. {ECO:0000255|PROSITE- ProRule:PRU00176} VAR_SEQ 154 154 V -> LCLHRVCHQGLRAGRRGAGPEPLPGPGHQGAAEKNQ LPWDQLHRPRGPSRTPRLQGGTLPPQRPPGQAPAQTTRAEP GPWKILTMVFTVLKGRPDFERGAGAWIRSKPLVLHPG (in isoform 3) /FTId=VSP_035211 VAR_SEQ 155 278 Missing (in isoform 3) /FTId=VSP_035212 VAR_SEQ 190 190 Y -> CCRKEPTSLGSAPQTAGAFEDTQAPGGHPSPTAASR AGPGSDHKGRTGPVENSHHGFHRIKGKTRF (in isoform 2) /FTId=VSP_035213 VAR_SEQ 191 278 Missing (in isoform 2) /FTId=VSP_035214 VAR_SEQ 267 278 ARGKFSPWFSPY -> TPAALTPLWARPLPWVVVSHVIK (in isoform 4). /FTId=VSP_040501 SEQUENCE 278 AA; 30386 MW; 4662DAB7022CACD5 CRC64; MWPFPSRSLF PPPTQAWLQT VSSDPEAQGW GAWNETKEIL GPEGGEGKEE KEEEEDAEED QDGDAGFLLS LLEQENLAEC PLPDQELEAI KMKVCAMEQA EGTPRPPGVQ QQAEEEEGTA AGQLLSPETV GCPLSGTPEE KVEADHRSVY VGNVDYGGSA EELEAHFSRC GEVHRVTILC DKFSGHPKGY AYIEFATKGS VQAAVELDQS LFRGRVIKVL PKRTNFPGIS STDRGGLRGH PGSRGAPFPH SGLQGRPRLR PQGQNRARGK FSPWFSPY
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$ 295.00
$ 99.00
Cat# AP11914c
(40 western blots)
Availability: In Stock
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