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NKX1-1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - NKX1-1 Antibody (Center) AP11916c
    NKX1-1 Antibody (Center) (Cat. #AP11916c) western blot analysis in SK-N-MC cell line lysates (35ug/lane).This demonstrates the NKX1-1 antibody detected the NKX1-1 protein (arrow).
  • IF - NKX1-1 Antibody (Center) AP11916c
    Fluorescent confocal image of Hela cell stained with NKX1-1 Antibody (Center)(Cat#AP11916c).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NKX1-1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NKX1-1 immunoreactivity is localized to Nucleus significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15270
Other Accession XP_931434.4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40659 Da
Antigen Region 1-1 aa
Additional Information
Other Names NK1 transcription factor-related protein 1, Homeobox protein 153, HPX-153, Homeobox protein SAX-2, NKX-11, NKX1-1, HPX153
Target/Specificity This NKX1-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the Central region of human NKX1-1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNKX1-1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX1-1
Synonyms HPX153
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}
Tissue Location Expressed in hemopoietic progenitor cells.
Research Areas
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The function of this protein remains unknown.

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Cat# AP11916c
(40 western blots)
Availability: Inquire
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