- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
SET07 Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP1191b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
SET07 Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | Q9NQR1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 42890 Da |
SET07 Antibody (C-term) - Additional info | |
| Gene ID 387893 | |
| Other Names SETD8; KMT5A; PRSET7; SET07; SET8; N-lysine methyltransferase SETD8; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; SET domain-containing protein 8 | |
| Target/Specificity This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364~393 amino acids from the C-terminal region of human SET07. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SET07 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SET07 Antibody (C-term) - Protein Information | |
| Name SETD8 | |
| Synonyms KMT5A, PRSET7, SET07, SET8 | |
| Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes | |
| Cellular Location Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin | |
SET07 Antibody (C-term) - Related products
AP1191a: SET07 Antibody (N-term)
AP1191b: SET07 Antibody (C-term)
AP6557c: SETD8 Antibody (Center)
RI14797: SETD8 predesign siRNA
DC07781: Human SETD8 cDNA Clone
LY11736a: SETD8 Over-expression Lysate
BP1191a: SET07 Antibody (N-term) Blocking Peptide
SET07 Antibody (C-term) - Application data
-
Western blot analysis of SET07 Antibody (C-term) (Cat. #AP1191b) in K562 cell line lysates (35ug/lane). SET07 (arrow) was detected using the purified Pab.
SET07 Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.
REFERENCES
Nishioka, K., et al., Mol. Cell 9(6):1201-1213 (2002). Fang, J., et al., Curr. Biol. 12(13):1086-1099 (2002).
