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SET07 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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Ordering Information
Catalog # SizeAvailability Price  
AP1191b 400 µl
(40 western blots)
In Stock
$ 265.00email & fax$ 251.75online
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AP1191b-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
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SET07 Antibody (C-term) - Product info

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary AccessionQ9NQR1
ReactivityHuman
Isotype Rabbit Ig
Calculated MW 42890 Da

SET07 Antibody (C-term) - Additional info

Gene ID 387893
Other Names
SETD8; KMT5A; PRSET7; SET07; SET8; N-lysine methyltransferase SETD8; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; SET domain-containing protein 8
Target/Specificity
This SET07 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-393 amino acids from the C-terminal region of human SET07.
Dilution
WB~~1:1000
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
SET07 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

SET07 Antibody (C-term) - Protein Information

Name SETD8
Synonyms KMT5A, PRSET7, SET07, SET8
Function
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Cellular Location
Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin

SET07 Antibody (C-term) - Related products

AM1191a: SET07 Antibody

AM1191b: SET07 Antibody

AP1191a: SET07 Antibody (N-term)

AP6557c: SETD8 Antibody (Center)

BP1191a: SET07 Antibody (N-term) Blocking Peptide

BP1191b: SET07 Antibody (C-term) Blocking Peptide

BP6557c: SETD8 Antibody (Center) Blocking Peptide

SET07 Antibody (C-term) - Research Areas

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.

REFERENCES

Nishioka, K., et al., Mol. Cell 9(6):1201-1213 (2002).
Fang, J., et al., Curr. Biol. 12(13):1086-1099 (2002).