|Application ||WB, E|
|Calculated MW||59765 Da|
|Antigen Region||42-69 aa|
|Other Names||Neuronal acetylcholine receptor subunit alpha-2, CHRNA2|
|Target/Specificity||This CHRNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-69 amino acids from the N-terminal region of human CHRNA2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CHRNA2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.|
|Cellular Location||Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence.
Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Rigbi, A., et al. Pharmacogenomics J. (2010) In press :
Hoda, J.C., et al. FEBS Lett. 583(10):1599-1604(2009)
Philibert, R.A., et al. Nicotine Tob. Res. 11(3):286-292(2009)
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