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GCLC Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP11943a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP11943a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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- Backgrounds
GCLC Antibody (N-term) - Product info | |
| Application | WB, IHC, IF, FC
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| Primary Accession | P48506 |
| Other Accession | NP_001489.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 72766 Da |
GCLC Antibody (N-term) - Additional info | |
| Gene ID 2729 | |
| Other Names GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase | |
| Target/Specificity This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human GCLC. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions GCLC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
GCLC Antibody (N-term) - Protein Information | |
| Name GCLC | |
| Synonyms GLCL, GLCLC | |
GCLC Antibody (N-term) - Related products
AP11943a: GCLC Antibody (N-term)
AP18483c: GCLC Antibody (Center)
DC05276: Human GCLC cDNA Clone
GCLC Antibody (N-term) - Application data
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GCLC Antibody (N-term) (Cat. #AP11943a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow).
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GCLC Antibody (N-term) (Cat. #AP11943a)immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GCLC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of GCLC Antibody (N-term)(Cat#AP11943a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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GCLC Antibody (N-term) (Cat. #AP11943a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
GCLC Antibody (N-term) - Research Areas
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BACKGROUND
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
REFERENCES
Man, B.L., et al. J Clin Neurosci 17(10):1244-1247(2010) Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) : Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010) Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010) Wang, X., et al. PLoS ONE 5 (8), E11934 (2010) :
