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>   home   >   Products   >   Primary Antibodies   >   TFIIIC90 Antibody (N-term)   

TFIIIC90 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - TFIIIC90 Antibody (N-term) AP11959a
    TFIIIC90 Antibody (N-term) (Cat. #AP11959a) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TFIIIC90 antibody detected the TFIIIC90 protein (arrow).
  • FC - TFIIIC90 Antibody (N-term) AP11959a
    TFIIIC90 Antibody (N-term) (Cat. #AP11959a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q9UKN8
Other Accession Q8BMQ2, NP_036336
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 91982 Da
Antigen Region 214-244 aa
Additional Information
Gene ID 9329
Other Names General transcription factor 3C polypeptide 4, TF3C-delta, Transcription factor IIIC 90 kDa subunit, TFIIIC 90 kDa subunit, TFIIIC90, Transcription factor IIIC subunit delta, GTF3C4
Target/Specificity This TFIIIC90 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-244 amino acids from the N-terminal region of human TFIIIC90.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFIIIC90 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GTF3C4
Function Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Cellular Location Nucleus.
Citations (0)

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Background

Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.

References

Joubert, B.R., et al. Genome Med 2 (3), 17 (2010) :
Dumay-Odelot, H., et al. J. Biol. Chem. 282(23):17179-17189(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Hsieh, Y.J., et al. Mol. Cell. Biol. 19(11):7697-7704(1999)

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Cat# AP11959a
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(40 western blots)
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