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TFAP4 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TFAP4 Antibody (Center) AP11969c
    TFAP4 Antibody (Center) (Cat. #AP11969c) western blot analysis in Hela,HL-60 cell line lysates (35ug/lane).This demonstrates the TFAP4 antibody detected the TFAP4 protein (arrow).
  • FC - TFAP4 Antibody (Center) AP11969c
    TFAP4 Antibody (Center) (Cat. #AP11969c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q01664
Other Accession NP_003214
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 125-154 aa
Additional Information
Other Names Transcription factor AP-4, Activating enhancer-binding protein 4, Class C basic helix-loop-helix protein 41, bHLHc41, TFAP4, BHLHC41
Target/Specificity This TFAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the Central region of human TFAP4.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFAP4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFAP4
Synonyms BHLHC41
Function Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'- CAGCTG-3'.
Cellular Location Nucleus. EMBL; S73885; AAB32235.1; -; mRNA EMBL; AC004653; AAC17116.1; -; Genomic_DNA EMBL; BC010576; AAH10576.1; -; mRNA EMBL; X57435; CAA40683.1; -; mRNA CCDS; CCDS10510.1; - PIR; I56893; I56893 RefSeq; NP_003214.1; NM_003223.2 UniGene; Hs.513305; - ProteinModelPortal; Q01664; - SMR; Q01664; - BioGrid; 112881; 115 DIP; DIP-53627N; - IntAct; Q01664; 25 MINT; Q01664; - STRING; 9606.ENSP00000204517; - iPTMnet; Q01664; - PhosphoSitePlus; Q01664; - BioMuta; TFAP4; - DMDM; 1729833; - EPD; Q01664; - MaxQB; Q01664; - PaxDb; Q01664; - PeptideAtlas; Q01664; - PRIDE; Q01664; - ProteomicsDB; 57980; - DNASU; 7023; - Ensembl; ENST00000204517; ENSP00000204517; ENSG00000090447 GeneID; 7023; - KEGG; hsa:7023; - UCSC; uc010uxg.3; human CTD; 7023; - DisGeNET; 7023; - EuPathDB; HostDB:ENSG00000090447.11; - GeneCards; TFAP4; - HGNC; HGNC:11745; TFAP4 HPA; HPA001912; - MIM; 600743; gene neXtProt; NX_Q01664; - OpenTargets; ENSG00000090447; - PharmGKB; PA36462; - eggNOG; KOG0561; Eukaryota eggNOG; ENOG4111JPE; LUCA GeneTree; ENSGT00390000015189; - HOGENOM; HOG000294087; - HOVERGEN; HBG061473; - InParanoid; Q01664; - KO; K09108; - OMA; HHVNVVT; - OrthoDB; EOG091G194I; - PhylomeDB; Q01664; - TreeFam; TF316489; - SIGNOR; Q01664; - GeneWiki; TFAP4; - GenomeRNAi; 7023; - PRO; PR:Q01664; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000090447; - CleanEx; HS_TFAP4; - ExpressionAtlas; Q01664; baseline and differential Genevisible; Q01664; HS GO; GO:0005739; C:mitochondrion; IDA:HPA GO; GO:0005634; C:nucleus; IDA:UniProtKB GO; GO:0017053; C:transcriptional repressor complex; ISS:UniProtKB GO; GO:0003677; F:DNA binding; IDA:UniProtKB GO; GO:0070888; F:E-box binding; IDA:UniProtKB GO; GO:0042826; F:histone deacetylase binding; IPI:UniProtKB GO; GO:0042803; F:protein homodimerization activity; IPI:UniProtKB GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB GO; GO:0003705; F:transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; IDA:UniProtKB GO; GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB GO; GO:0001077; F:transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding; IEA:Ensembl GO; GO:0071549; P:cellular response to dexamethasone stimulus; IEA:Ensembl GO; GO:0006978; P:DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; IDA:UniProtKB GO; GO:0043922; P:negative regulation by host of viral transcription; IDA:UniProtKB GO; GO:0071157; P:negative regulation of cell cycle arrest; IMP:UniProtKB GO; GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB GO; GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:UniProtKB GO; GO:0043392; P:negative regulation of DNA binding; IDA:UniProtKB GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB GO; GO:0043923; P:positive regulation by host of viral transcription; IDA:UniProtKB GO; GO:0043065; P:positive regulation of apoptotic process; IDA:UniProtKB GO; GO:2001269; P:positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; IEA:Ensembl GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB GO; GO:0065003; P:protein-containing complex assembly; IDA:UniProtKB GO; GO:1901990; P:regulation of mitotic cell cycle phase transition; IDA:UniProtKB CDD; cd00083; HLH; 1 Gene3D; 4.10.280.10; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf Pfam; PF00010; HLH; 1 SMART; SM00353; HLH; 1 SUPFAM; SSF47459; SSF47459; 1 PROSITE; PS50888; BHLH; 1 1: Evidence at protein level; Activator; Complete proteome; Direct protein sequencing; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation CHAIN 1 338 Transcription factor AP-4 /FTId=PRO_0000127458 DOMAIN 48 99 bHLH. {ECO:0000255|PROSITE- ProRule:PRU00981} REGION 100 120 Leucine-zipper 1 REGION 151 179 Leucine-zipper 2 COMPBIAS 193 222 Gln-rich COMPBIAS 225 244 Pro-rich MOD_RES 123 123 Phosphoserine MOD_RES 124 124 Phosphoserine MOD_RES 139 139 Phosphoserine CROSSLNK 147 147 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) CROSSLNK 187 187 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) CROSSLNK 189 189 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) CROSSLNK 285 285 Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) VARIANT 218 218 Q -> H (in dbSNP:rs251732) /FTId=VAR_059346 SEQUENCE 338 AA; 38726 MW; 540C008658596B83 CRC64; MEYFMVPTQK VPSLQHFRKT EKEVIGGLCS LANIPLTPET QRDQERRIRR EIANSNERRR MQSINAGFQS LKTLIPHTDG EKLSKAAILQ QTAEYIFSLE QEKTRLLQQN TQLKRFIQEL SGSSPKRRRA EDKDEGIGSP DIWEDEKAED LRREMIELRQ QLDKERSVRM MLEEQVRSLE AHMYPEKLKV IAQQVQLQQQ QEQVRLLHQE KLEREQQQLR TQLLPPPAPT HHPTVIVPAP PPPPSHHINV VTMGPSSVIN SVSTSRQNLD TIVQAIQHIE GTQEKQELEE EQRRAVIVKP VRSCPEAPTS DTASDSEASD SDAMDQSREE PSGDGELP
Research Areas
Citations (0)

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Background

Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).

References

Ku, W.C., et al. Mol. Cell Proteomics 8(9):2034-2050(2009)
Cao, J., et al. Int. J. Surg. Pathol. 17(1):16-21(2009)
Jung, P., et al. Proc. Natl. Acad. Sci. U.S.A. 105(39):15046-15051(2008)
Pons, J., et al. Biochemistry 47(1):14-29(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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Cat# AP11969c
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