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SAE1 (AOS1) Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1199b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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SAE1 (AOS1) Antibody (C-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9UBE0 |
| Other Accession | NP_005491 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 38450 Da |
SAE1 (AOS1) Antibody (C-term) - Additional info | |
| Gene ID 10055 | |
| Other Names SAE1; AOS1; SUA1; UBLE1A; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A | |
| Target/Specificity This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272~302 amino acids from the C-terminal region of human SAE1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SAE1 (AOS1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SAE1 (AOS1) Antibody (C-term) - Protein Information | |
| Name SAE1 | |
| Synonyms AOS1, SUA1, UBLE1A | |
| Function The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2 | |
| Cellular Location Nucleus. | |
| Tissue Location Expression level increases during S phase and drops in G2 phase (at protein level) | |
SAE1 (AOS1) Antibody (C-term) - Related products
AP1199a: SAE1 (AOS1) Antibody (N-term)
AP1199b: SAE1 (AOS1) Antibody (C-term)
AP2011b: SAE1 (AOS1) Antibody (C-term)
AP2511a: SAE1 (AOS1) Antibody (N-term)
AP2511b: SAE1 (AOS1) Antibody (C-term)
DC00151: Human SAE1 cDNA Clone
LY10831a: SAE1 Over-expression Lysate
BP1199a: SAE1 Antibody (N-term) Blocking Peptide
BP1199b: SAE1 Antibody (C-term) Blocking Peptide
BP2011b: SAE1 Antibody (C-term) Blocking Peptide
BP2511a: AOS1 Antibody (N-term) Blocking Peptide
BP2511b: AOS1 Antibody (C-term) Blocking Peptide
SAE1 (AOS1) Antibody (C-term) - Application data
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Western blot analysis of SAE1-S279 (RB02786) in mouse testis tissue lysates (35ug/lane).SAE1 (arrow) was detected using the purified Pab.
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Western blot analysis of SAE1 (arrow) using rabbit polyclonal SAE1 Antibody (C-term)(Cat.#AP1199b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SAE1 gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SAE1 (AOS1) Antibody (C-term) - Research Areas
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BACKGROUND
Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respects to size and ubiquity ?to which eukaryotic proteins are subject. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied for over two decades. Recently a new group of ubiquitin-like (Ubl) proteins have come to light. One of the most intriguing of them is SUMO (small ubiquitin-like modifier, ~12kDa) also known as Sentrin. SUMO family has been described in vertebrates: SUMO-1 and the closest homologs SUMO-2 and SUMO-3. SUMO have been shown to bind and regulate mammalian SP-RINGs (such as Mdm2, PIAS and PML), RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, Drosophila Ttk69, Dorsal, CaMK, yeast Septins, and viral CMV-IE1/2, EBV-BZLF1, HPV/BPV-E1. These bindings implicate SUMO in the stabilization of the target proteins and/or their localization to subcellular complexes. SUMO research enters now an exciting phase with a promise to help understanding how cells orchestrate the complexities of rapidly regulating protein level and activity.
REFERENCES
Desterro, J.M., et al., J. Biol. Chem. 274(15):10618-10624 (1999). Gong, L., et al., FEBS Lett. 448(1):185-189 (1999). Okuma, T., et al., Biochem. Biophys. Res. Commun. 254(3):693-698 (1999).
