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C18orf24 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | ||||
| AP12001b | DISCONTINED INQUIRE CLICK INQUIRE Add to cart | |||
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
C18orf24 Antibody (C-term) - Product info | |
| Application | WB, IHC, IF, FC
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| Primary Accession | Q96BD8 |
| Other Accession | NP_659497.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 29484 Da |
C18orf24 Antibody (C-term) - Additional info | |
| Gene ID 220134 | |
| Other Names SKA1; C18orf24; Spindle and kinetochore-associated protein 1 | |
| Target/Specificity This C18orf24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-217 amino acids from the C-terminal region of human C18orf24. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions C18orf24 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
C18orf24 Antibody (C-term) - Protein Information | |
| Name SKA1 | |
| Synonyms C18orf24 | |
| Function Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules | |
| Cellular Location Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. Localizes to both the mitotic spindle and kinetochore-associated proteins. Associates with kinetochores following microtubule attachment from prometaphase, through mid- anaphase and then vanishes in telophase | |
C18orf24 Antibody (C-term) - Related products
C18orf24 Antibody (C-term) - Application data
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C18orf24 Antibody (C-term) (Cat. #AP12001b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C18orf24 antibody detected the C18orf24 protein (arrow).
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C18orf24 Antibody (C-term) (Cat. #AP12001b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of C18orf24 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Confocal immunofluorescent analysis of C18orf24 Antibody (C-term)(Cat#AP12001b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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C18orf24 Antibody (C-term) (Cat. #AP12001b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
C18orf24 Antibody (C-term) - Research Areas
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BACKGROUND
Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.
REFERENCES
Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010) Welburn, J.P., et al. Dev. Cell 16(3):374-385(2009) Hanisch, A., et al. EMBO J. 25(23):5504-5515(2006) Sauer, G., et al. Mol. Cell Proteomics 4(1):35-43(2005)
