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DEPD5 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DEPD5 Antibody (C-term) AP12010B
    All lanes : Anti-DEPD5 Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 171 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - DEPD5 Antibody (C-term) AP12010B
    Overlay histogram showing Hela cells stained with AP12010b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP12010b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession O75140-1
Other Accession P61460, NP_001007189.1, O75140
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 1404-1432 aa
Additional Information
Other Names DEP domain-containing protein 5, DEPDC5, KIAA0645
Target/Specificity This DEPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1404-1432 amino acids of human DEPD5.
Dilution WB~~1:2000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDEPD5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations ( 0 )

References

Seng, T.J., et al. Genes Chromosomes Cancer 43(2):181-193(2005)
Dunham, I., et al. Nature 402(6761):489-495(1999)

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$ 365.00
$ 140.00
Cat# AP12010B
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