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DEPD5 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • FC - DEPD5 Antibody  (C-term) AP12010b
    Overlay histogram showing Hela cells stained with AP12010b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP12010b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - DEPD5 Antibody  (C-term) AP12010b
    All lanes : Anti-DEPD5 Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 171 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - DEPD5 Antibody  (C-term) AP12010b
    Western blot analysis of DEPD5 Antibody (C-term) Pab (Cat. #AP12010b) pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. DEPD5 Antibody (C-term) (arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75140
Other Accession P61460, NP_001007189.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 181264 Da
Antigen Region 1404-1432 aa
Additional Information
Gene ID 9681
Other Names DEP domain-containing protein 5, DEPDC5, KIAA0645
Target/Specificity This DEPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1404-1432 amino acids from the C-terminal region of human DEPD5.
Dilution FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDEPD5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0645
Function As a component of the GATOR1 complex, inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC- containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling.
Cellular Location Cytoplasm, cytosol. Cytoplasm, perinuclear region
Tissue Location Expressed in developing and adult brain.
Research Areas
Citations (0)

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Seng, T.J., et al. Genes Chromosomes Cancer 43(2):181-193(2005)
Dunham, I., et al. Nature 402(6761):489-495(1999)

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$ 295.00
$ 99.00
Cat# AP12010b
(40 western blots)
Availability: In Stock
Bulk Size
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