|Application ||WB, IHC-P, FC, IF, E|
|Calculated MW||38865 Da|
|Antigen Region||165-193 aa|
|Other Names||Inactive caspase-12, CASP-12, CASP12|
|Target/Specificity||This CASP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-193 amino acids from the Central region of human CASP12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CASP12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF.|
|Tissue Location||Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis|
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Provided below are standard protocols that you may find useful for product applications.
Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene.
Lee, H.J., et al. Arch. Biochem. Biophys. 502(1):68-73(2010)
Plantinga, T.S., et al. J. Acquir. Immune Defic. Syndr. 55(1):87-94(2010)
McCall, M.B., et al. Eur. Cytokine Netw. 21(2):77-83(2010)
Kachapati, K., et al. Hum. Mutat. 27 (9), 975 (2006) :
Xue, Y., et al. Am. J. Hum. Genet. 78(4):659-670(2006)
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