|Application ||IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||188915 Da|
|Antigen Region||245-573 aa|
|Other Names||PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger protein RIZ, PRDM2, KMT8, RIZ|
|Target/Specificity||Purified recombinant GST fusion protein encoding aa 245-573 of rat Riz1. This antibody also recognizes the isoform beta (Riz2) of PRDM2 (185 kDa).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRDM2 (RIZ1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.|
|Tissue Location||Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen Isoform 1 is expressed in testis at much higher level than isoform 3.|
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Provided below are standard protocols that you may find useful for product applications.
PRDM2 is a 250-kD nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. Using fluorescence in situ hybridization, the PRDM2 gene has been mapped to 1p36. PRDM2 may be a candidate target of 1p36 alterations that commonly occur in neuroendocrine, breast, liver, colon, skin, and lymphoid tumors.
Muraosa, Y., et al., Eur. J. Biochem. 235(3):471-479 (1996). Buyse, I.M., et al., Proc. Natl. Acad. Sci. U.S.A. 92(10):4467-4471 (1995).
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