PURB Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, FC, E |
---|---|
Primary Accession | Q96QR8 |
Other Accession | Q68A21, O35295, NP_150093.1 |
Reactivity | Human |
Predicted | Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 33241 Da |
Antigen Region | 260-289 aa |
Gene ID | 5814 |
---|---|
Other Names | Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B, PURB |
Target/Specificity | This PURB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the C-terminal region of human PURB. |
Dilution | IF~~1:10~50 WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PURB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PURB |
---|---|
Function | Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity). |
Cellular Location | Nucleus. |
Tissue Location | Expressed in myocardium of heart failure patients. |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.
References
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Ramsey, J.E., et al. Biochemistry 48(27):6348-6360(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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