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>   home   >   Products   >   Primary Antibodies   >   PURB Antibody (C-term)   

PURB Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - PURB Antibody (C-term) AP12051B
    Fluorescent image of A549 cell stained with PURB Antibody (C-term)(Cat#AP12051b).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PURB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).PURB immunoreactivity is localized to Nucleus significantly.
  • WB - PURB Antibody (C-term) AP12051B
    PURB Antibody (C-term) (Cat. #AP12051b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PURB antibody detected the PURB protein (arrow).
  • FC - PURB Antibody (C-term) AP12051B
    PURB Antibody (C-term) (Cat. #AP12051b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, E
Primary Accession Q96QR8
Other Accession Q68A21, O35295, NP_150093.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 33241 Da
Antigen Region 260-289 aa
Additional Information
Gene ID 5814
Other Names Transcriptional activator protein Pur-beta, Purine-rich element-binding protein B, PURB
Target/Specificity This PURB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the C-terminal region of human PURB.
Dilution IF~~1:10~50
WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPURB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PURB
Function Has capacity to bind repeated elements in single- stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine- rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed in myocardium of heart failure patients.
Citations (0)

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Background

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.

References

Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Ramsey, J.E., et al. Biochemistry 48(27):6348-6360(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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Discontinued
Cat# AP12051B
Size:
(40 western blots)

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