|Application ||WB, E|
|Other Accession||Q90655, NP_733796.2, NP_003235.1|
|Calculated MW||43013 Da|
|Antigen Region||145-174 aa|
|Other Names||Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1, TGIF1, TGIF|
|Target/Specificity||This TGIF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human TGIF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TGIF1 Antibody (Center A160) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII- RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and eight variants, encoding four distinct isoforms, are described.
Bengoechea-Alonso, M.T., et al. Oncogene 29(38):5322-5328(2010)
Paulussen, A.D., et al. Eur. J. Hum. Genet. 18(9):999-1005(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Demange, C., et al. Mol. Cell 36(6):1073-1085(2009)
Hamid, R., et al. Mol Oncol 3 (5-6), 451-463 (2009) :
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.