|Application ||WB, E|
|Calculated MW||28518 Da|
|Antigen Region||13-39 aa|
|Other Names||Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase, ELANE, ELA2|
|Target/Specificity||This Neutrophil elastase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-39 amino acids from the N-terminal region of human Neutrophil elastase.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Neutrophil elastase Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.|
|Tissue Location||Bone marrow cells.|
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Provided below are standard protocols that you may find useful for product applications.
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
Kallquist, L., et al. Exp. Cell Res. 316(19):3182-3196(2010)
Rabai, G., et al. Thromb. Res. 126 (2), E94-E101 (2010) :
Newburger, P.E., et al. Pediatr Blood Cancer 55(2):314-317(2010)
Hayashi, M., et al. J Nippon Med Sch 77(2):80-85(2010)
Hector, A., et al. Mediators Inflamm. 2010, 809591 (2010) :
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