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CYBA Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CYBA Antibody (C-term) AP12149b
    CYBA Antibody (C-term) (Cat. #AP12149b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the CYBA antibody detected the CYBA protein (arrow).
  • IF - CYBA Antibody (C-term) AP12149b
    Confocal immunofluorescent analysis of CYBA Antibody (C-term)(Cat#AP12149b) with NCI-H292 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13498
Other Accession NP_000092.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21013 Da
Antigen Region 117-145 aa
Additional Information
Gene ID 1535
Other Names Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox, p22phox, CYBA
Target/Specificity This CYBA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the C-terminal region of human CYBA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYBA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Cellular Location Cell membrane
Research Areas
Citations (0)

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Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Katakami, N., et al. Atherosclerosis 212(2):534-538(2010)
Tu, Y.C., et al. Acta Pharmacol. Sin. 31(7):874-880(2010)
Moreno, M.U., et al. Drug News Perspect. 23(5):316-324(2010)
Cross, D.S., et al. BMC Genet. 11, 51 (2010) :

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Cat# AP12149b
(40 western blots)
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