- CITATIONS: 4
|Application ||WB, IHC-P, IF, E|
|Calculated MW||64062 Da|
|Antigen Region||1-30 aa|
|Other Names||PR domain zinc finger protein 14, 211-, PR domain-containing protein 14, PRDM14|
|Target/Specificity||This PRDM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRDM14.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRDM14 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.|
|Tissue Location||Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level)|
Provided below are standard protocols that you may find useful for product applications.
Similar to acetylation and phosphorylation, histone methylation at the N terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET domain containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET domain family, and another 17 make up the PR domain family that is related to the SET domain family. PRDM14 is part of a family of PR domain genes that are involved in tumorigenesis. It may function as a transcription factor.
Xiao, B., et al., Curr. Opin. Struct. Biol. 13(6):699-705 (2003).
Dias Neto, E., et al., Proc. Natl. Acad. Sci. U.S.A. 97(7):3491-3496 (2000).
Jiang, G.L., et al., Histol. Histopathol. 15(1):109-117 (2000).
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