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>   home   >   Products   >   Primary Antibodies   >   CPSF7 Antibody (C-term)   

CPSF7 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CPSF7 Antibody (C-term) AP12151B
    CPSF7 Antibody (C-term) (Cat. #AP12151b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CPSF7 antibody detected the CPSF7 protein (arrow).
  • FC - CPSF7 Antibody (C-term) AP12151B
    CPSF7 Antibody (C-term) (Cat. #AP12151b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession Q8N684
Other Accession Q5XI29, Q8BTV2, NP_001129512.1, NP_001136037.1, NP_079087.3
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 52050 Da
Antigen Region 427-455 aa
Additional Information
Gene ID 79869
Other Names Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59, CPSF 59 kDa subunit, Pre-mRNA cleavage factor Im 59 kDa subunit, CPSF7
Target/Specificity This CPSF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 427-455 amino acids from the C-terminal region of human CPSF7.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCPSF7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CPSF7 (HGNC:30098)
Function Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:8626397, PubMed:17024186, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'- end, so called cleavage and polyadenylation signals (pA signals) (PubMed:8626397, PubMed:17024186). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'- untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085). CPSF7 activates directly the mRNA 3'-processing machinery (PubMed:29276085). Binds to pA signals in RNA substrates (PubMed:8626397, PubMed:17024186).
Cellular Location Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460).
Citations (0)

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Background

CPSF7 is probable a component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.

References

Olsen, J.V., et al. Cell 127(3):635-648(2006)
Lim, J., et al. Cell 125(4):801-814(2006)
Brill, L.M., et al. Anal. Chem. 76(10):2763-2772(2004)
Zhou, Z., et al. Nature 419(6903):182-185(2002)
Ruegsegger, U., et al. J. Biol. Chem. 271(11):6107-6113(1996)

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$ 295.00
$ 99.00
Cat# AP12151B
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Quantity:
(40 western blots)
Availability: In Stock
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