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NKD2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - NKD2 Antibody (C-term) AP12158B
    Confocal immunofluorescent analysis of NKD2 Antibody (C-term)(Cat#AP12158b) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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  • WB - NKD2 Antibody (C-term) AP12158B
    NKD2 Antibody (C-term) (Cat. #AP12158b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NKD2 antibody detected the NKD2 protein (arrow).
    detail
  • IHC-P - NKD2 Antibody (C-term) AP12158B
    NKD2 Antibody (C-term) (Cat. #AP12158b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NKD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - NKD2 Antibody (C-term) AP12158B
    NKD2 Antibody (C-term) (Cat. #AP12158b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IF, IHC-P, WB, E
Primary Accession Q969F2
Other Accession NP_149111.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 50055 Da
Antigen Region 366-393 aa
Additional Information
Gene ID 85409
Other Names Protein naked cuticle homolog 2, Naked-2, hNkd2, NKD2
Target/Specificity This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the C-terminal region of human NKD2.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNKD2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKD2
Function Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
Cellular Location Cell membrane. Cytoplasm. Cytoplasmic vesicle
Tissue Location Expressed in kidney, lung, pancreas and spleen.
Research Areas
Citations (0)
citation

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Background

In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.

References

Hu, T., et al. J. Biol. Chem. 285(18):13561-13568(2010)
Ding, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(36):13433-13438(2008)
Li, C., et al. Mol. Biol. Cell 18(8):3081-3093(2007)
Adamowicz, M., et al. Genes Chromosomes Cancer 45(9):829-838(2006)
Li, C., et al. Proc. Natl. Acad. Sci. U.S.A. 101(15):5571-5576(2004)

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$ 365.00
$ 140.00
Cat# AP12158B
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