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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PCDHB3 Antibody (N-term)   

PCDHB3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PCDHB3 Antibody (N-term) AP12159a
    PCDHB3 Antibody (N-term) (Cat. #AP12159a) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PCDHB3 antibody detected the PCDHB3 protein (arrow).
  • IHC-P - PCDHB3 Antibody (N-term) AP12159a
    PCDHB3 Antibody (N-term) (Cat. #AP12159a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDHB3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - PCDHB3 Antibody (N-term) AP12159a
    Confocal immunofluorescent analysis of PCDHB3 Antibody (N-term)(Cat#AP12159a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9Y5E6
Other Accession NP_061760.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 12-39 aa
Additional Information
Other Names Protocadherin beta-3, PCDH-beta-3, PCDHB3
Target/Specificity This PCDHB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human PCDHB3.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCDHB3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCDHB3
Function Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
Cellular Location Cell membrane; Single-pass type I membrane protein EMBL; AF152496; AAD43757.1; -; mRNA EMBL; AF217755; AAK51622.1; -; mRNA EMBL; AK313451; BAG36239.1; -; mRNA EMBL; CH471062; EAW61981.1; -; Genomic_DNA CCDS; CCDS4245.1; - RefSeq; NP_061760.1; NM_018937.4 UniGene; Hs.508987; - UniGene; Hs.674730; - UniGene; Hs.708778; - ProteinModelPortal; Q9Y5E6; - SMR; Q9Y5E6; - BioGrid; 121072; 19 STRING; 9606.ENSP00000231130; - iPTMnet; Q9Y5E6; - PhosphoSitePlus; Q9Y5E6; - BioMuta; PCDHB3; - DMDM; 13431377; - EPD; Q9Y5E6; - PaxDb; Q9Y5E6; - PeptideAtlas; Q9Y5E6; - PRIDE; Q9Y5E6; - ProteomicsDB; 86345; - Ensembl; ENST00000231130; ENSP00000231130; ENSG00000113205 GeneID; 56132; - KEGG; hsa:56132; - UCSC; uc003lio.5; human CTD; 56132; - EuPathDB; HostDB:ENSG00000113205.5; - GeneCards; PCDHB3; - H-InvDB; HIX0032374; - HGNC; HGNC:8688; PCDHB3 HPA; HPA050296; - MIM; 604967; gene MIM; 606329; gene neXtProt; NX_Q9Y5E6; - PharmGKB; PA33037; - eggNOG; KOG3594; Eukaryota eggNOG; ENOG410XQHI; LUCA HOGENOM; HOG000220892; - HOVERGEN; HBG054878; - InParanoid; Q9Y5E6; - KO; K16494; - OrthoDB; EOG091G024L; - PhylomeDB; Q9Y5E6; - TreeFam; TF332299; - GeneWiki; PCDHB3; - GenomeRNAi; 56132; - PRO; PR:Q9Y5E6; - Proteomes; UP000005640; Chromosome 5 Bgee; ENSG00000113205; - CleanEx; HS_PCDHB3; - ExpressionAtlas; Q9Y5E6; baseline and differential Genevisible; Q9Y5E6; HS GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc GO; GO:0005509; F:calcium ion binding; IEA:InterPro GO; GO:0016339; P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules; NAS:UniProtKB GO; GO:0007155; P:cell adhesion; TAS:ProtInc GO; GO:0007268; P:chemical synaptic transmission; TAS:UniProtKB GO; GO:0007156; P:homophilic cell adhesion via plasma membrane adhesion molecules; IEA:InterPro GO; GO:0007399; P:nervous system development; TAS:ProtInc GO; GO:0007416; P:synapse assembly; TAS:UniProtKB InterPro; IPR002126; Cadherin InterPro; IPR015919; Cadherin-like InterPro; IPR032455; Cadherin_C InterPro; IPR020894; Cadherin_CS InterPro; IPR013164; Cadherin_N InterPro; IPR030735; PCDHB2/3 PANTHER; PTHR24028:SF77; PTHR24028:SF77; 1 Pfam; PF00028; Cadherin; 5 Pfam; PF08266; Cadherin_2; 1 Pfam; PF16492; Cadherin_C_2; 1 PRINTS; PR00205; CADHERIN SMART; SM00112; CA; 6 SUPFAM; SSF49313; SSF49313; 6 PROSITE; PS00232; CADHERIN_1; 5 PROSITE; PS50268; CADHERIN_2; 6 2: Evidence at transcript level; Calcium; Cell adhesion; Cell membrane; Complete proteome; Glycoprotein; Membrane; Polymorphism; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix SIGNAL 1 26 CHAIN 27 796 Protocadherin beta-3 /FTId=PRO_0000003918 TOPO_DOM 27 690 Extracellular. TRANSMEM 691 711 Helical. TOPO_DOM 712 796 Cytoplasmic. DOMAIN 35 133 Cadherin 1. {ECO:0000255|PROSITE- ProRule:PRU00043} DOMAIN 138 242 Cadherin 2. {ECO:0000255|PROSITE- ProRule:PRU00043} DOMAIN 247 347 Cadherin 3. {ECO:0000255|PROSITE- ProRule:PRU00043} DOMAIN 352 451 Cadherin 4. {ECO:0000255|PROSITE- ProRule:PRU00043} DOMAIN 456 561 Cadherin 5. {ECO:0000255|PROSITE- ProRule:PRU00043} DOMAIN 568 671 Cadherin 6. {ECO:0000255|PROSITE- ProRule:PRU00043} CARBOHYD 169 169 N-linked (GlcNAc...) asparagine CARBOHYD 418 418 N-linked (GlcNAc...) asparagine CARBOHYD 436 436 N-linked (GlcNAc...) asparagine CARBOHYD 567 567 N-linked (GlcNAc...) asparagine VARIANT 41 41 K -> R (in dbSNP:rs31849) /FTId=VAR_024391 VARIANT 207 207 E -> K (in dbSNP:rs12515688) /FTId=VAR_033703 VARIANT 414 414 R -> S (in dbSNP:rs3733699) /FTId=VAR_020366 SEQUENCE 796 AA; 86773 MW; F586CF1BC3A2B0D2 CRC64; MEAGGERFLR QRQVLLLFVF LGGSLAGSES RRYSVAEEKE KGFLIANLAK DLGLRVEELA ARGAQVVSKG NKQHFQLSHQ TGDLLLNEKL DREELCGPTE PCILHFQILL QNPLQFVTNE LRIIDVNDHS PVFFENEMHL KILESTLPGT VIPLGNAEDL DVGRNSLQNY TITPNSHFHV LTRSRRDGRK YPELVLDKAL DREEQPELSL TLTALDGGSP PRSGTAQINI QVLDINDNAP EFAQPLYEVA VLENTPVNSV IVTVSASDLD TGSFGTISYA FFHASEEIRK TFQLNPITGD MQLVKYLNFE AINSYEVDIE AKDGGGLSGK STVIVQVVDV NDNPPELTLS SVNSPIPENS GETVLAVFSV SDLDSGDNGR VMCSIENNLP FFLKPSVENF YTLVSEGALD RETRSEYNIT ITITDLGTPR LKTKYNITVL VSDVNDNAPA FTQISYTLFV RENNSPALHI GSVSATDRDS GTNAQVTYSL LPPQDPHLPL SSLVSINADN GHLFALRSLD YEALQAFEFR VGATDRGSPA LSSEALVRVL VLDANDNSPF VLYPLQNGSA PCTELVPRAA EPGYLVTKVV AVDGDSGQNA WLSYQLLKAT EPGLFGVWAH NGEVRTARLL SERDAAKHRL VVLVKDNGEP PRSATATLHV LLVDGFSQPY LPLPEAAPAQ AQADLLTVYL VVALASVSSL FLFSVLLFVA VRLCRRSRAA SVGRCSVPEG PFPGQMVDVS GTGTLSQSYQ YEVCLTGGSG TNEFKFLKPI IPNFVAQGAE RVSEANPSFR KSFEFS
Research Areas
Citations (0)

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Background

This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections.

References

Frank, M., et al. Curr. Opin. Cell Biol. 14(5):557-562(2002)
Vanhalst, K., et al. FEBS Lett. 495 (1-2), 120-125 (2001) :
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)

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$ 295.00
$ 99.00
Cat# AP12159a
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