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PCDHA9 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PCDHA9 Antibody (N-term) AP12165a
    PCDHA9 Antibody (N-term) (Cat. #AP12165a) western blot analysis in NCI-H460,K562 cell line lysates (35ug/lane).This demonstrates the PCDHA9 antibody detected the PCDHA9 protein (arrow).
    detail
  • FC - PCDHA9 Antibody (N-term) AP12165a
    PCDHA9 Antibody (N-term) (Cat. #AP12165a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q9Y5H5
Other Accession Q9UN72, NP_054724.1, NP_114063.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 102402 Da
Antigen Region 226-253 aa
Additional Information
Gene ID 9752
Other Names Protocadherin alpha-9, PCDH-alpha-9, PCDHA9, KIAA0345
Target/Specificity This PCDHA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-253 amino acids from the N-terminal region of human PCDHA9.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCDHA9 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCDHA9
Synonyms KIAA0345
Function Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
citation

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Background

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

References

Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Wu, Q., et al. Genome Res. 11(3):389-404(2001)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)

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$ 365.00
$ 140.00
Cat# AP12165a
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